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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Immunol. 2012 Aug 24;189(7):3421–3429. doi: 10.4049/jimmunol.1201224

Figure 4. IL-33 expression in mast cells is dependent on PI3K activity.

Figure 4

BMMCs were pretreated with different doses of PI3K inhibitors, LY294002 and wortmannin, 30 minutes prior to IgE/DNP (A and C, black bar) and 0.25μM ionomycin (C, gray bar) activation. IL-33 expression was analyzed by real-time PCR. (B) BMMCs pretreated with 10μM LY294002 or 100nM Wortmannin were activated with IgE/DNP for 24 hours. IL-33 protein expression was detected by intracellular staining. Gray line, isotype control; black dot, unstimulated; gray fill, IgE/DNP stimulation; black line, LY294002 treatment; black dash, wortmannin treatment. n=3 from 2-3 individual experiments; *p<0.05, **p<0.01, ***p<0.001, n.s. = no significant difference.