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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: Dev Biol. 2012 Sep 19;372(2):166–177. doi: 10.1016/j.ydbio.2012.09.006

Figure 2. FGF4 is sufficient to induce ectopic expression of Pax7 in the non-neural ectoderm (NNE).

Figure 2

A–O) Mixes containing FGF4 induce ectopic expression of Pax7 (A, G, J), while those that do not contain FGF4 do not induce Pax7 (D, M). B, H, K) Ectopic Pax7 expression can occur in the absence of mesoderm (Bra). J–L) Ectopic Pax7 is induced by FGF4 in the absence of N2 supplement. M–O) N2 supplement alone does not induce either marker. Boxed areas in whole mounts indicate magnified regions shown below each sample, and dashed lines indicate plane of corresponding sections. Samples showing ectopic Pax7+/Bra− expression are indicated (“n”). P) In mixes that contain FGF4, 10% or more of beads induce ectopic expression of Pax7 in the absence of Bra (red bars). Using FGF4 alone (without inclusion of N2) greatly increased the frequency of total Pax7 expression (red and blue bars combined), while Pax7 alone (red bar only) is seen in 10% of beads. Scale bars are 200µm (A, D, G, J, M) and 100µm (B, C, E, F, H, I, K, L, N, O).