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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: Dev Biol. 2012 Sep 19;372(2):166–177. doi: 10.1016/j.ydbio.2012.09.006

Figure 6. Bmp4 expression is upregulated by FGF4.

Figure 6

Using in situ hybridization, Bmp4 can be detected as early at 4 hours (A, B), 6 hours (C, D) and 16 hours (E, F) after FGF4 application. Dashed lines in A, C, and D indicate the plane of sections shown below. Further analysis of panel F displaying DAPI nuclear staining (G), Pax7+ (H arrowheads) and Bra− (I) immunofluorescence. A section from caudal region of embryo E displays endogenous signal for BMP4 (J, black arrowhead) and Pax7 (K, green arrowhead) in the neural folds, and Bra (L, red arrowhead) in the notochord. Scale bars are 200µm (A, C, E), 100µm (B, D, F) and 50µm (G–L).