Table 1.

Relative amount of sterols (% of total sterols) in different A. fumigatus wild-type (CM-237), cyp51 deleted strains (Δcyp51A Δcyp51B), and azole resistance mutants strains with different cyp51A point mutations (CM-796:G54V; CM-3269:TRL98H; CM-2159: M220K).

Sterol names	Strains
	CM-237*	Δcyp51A	Δcyp51B	CM-796*	CM-3269*	CM-2159*
1) 24-methylcholesta-5,7,9(11),22-tetraen-3β-ol	1.09	3.69	2.74	0.85	1.18	0.92
2) 24-methylcholesta-5,8,22-trien-3β-ol	0.91	0.87	0.77	0.90	0.80	0.85
3) 24-methylcholesta-5,7,9,22-tetraen-3β-ol	0.71	5.69	4.57	0.00	0.00	0.00
4) 24-methylcholesta-5,7,22-trien-3β-ol (Ergosterol)	78.12	59.48	48.82	84.08	83.70	88.44
5) 24-methylcholesta-7,22 (28)-dien-3β-ol	2.62	1.84	1.18	1.51	1.67	1.09
6) 24-methylcholesta-5,7,22,24(28)-tetraen-3β-ol	0.48	0.41	0.25	0.32	0.21	0.28
7) 24-methylcholesta-7,22,24(28)-trien-3β-ol	0.66	1.44	0.71	0.82	0.51	0.20
8) 24-methylcholesta-5,7,24(28)-trien-3β-ol	0.91	0.14	0.34	0.08	0.19	0.16
9) 24-methylcholesta-7,24(28)-dien-3β-ol (Episterol)	1.51	2.55	2.01	3.59	1.84	1.08
10) 24-Ethylcholesta-5,7,22-trien-3β-ol	7.48	5.48	9.26	2.43	6.46	5.10
11) 4,4,14-trimethylcholesta-8,24-dien-3β-ol (Lanosterol)	1.06	1.39	2.87	3.63	0.29	0.18
12) 4α,24-dimethylcholesta-8,24(28)-dien-3β-ol	0.99	2.03	0.98	0.73	0.00	0.82
13) 4,4,14,24-tetramethylcholesta-8,24(28)-dien-3β-ol (Eburicol)	1.14	9.54	21.95	0.41	1.30	0.23
14) 4,4,24-trimethylcholesta-8,24(28)-dien-3β-ol	2.04	5.44	3.56	0.65	0.62	0.65

^*Geometric mean of MICs to antifungal agents of A. fumigatus strains: CM-237: ITC (0.25 μg/ml), VRC (0.48 μg/ml), POS (0.06 μg/ml), FLC (1094.55 μg/ml), AMB (0.26 μg/ml), TRB (5.4 μg/ml); CM-796: ITC (16 μg/ml), VRC (1.19 μg/ml), POS (1.68 μg/ml), FLC (1094.55 μg/ml), AMB (0.15 μg/ml), TRB (2.38 μg/ml; CM-2159: ITC (16 μg/ml), VRC (1.19 μg/ml), POS (2 μg/ml), FLC (1094.55 μg/ml), AMB (0.3 μg/ml), TRB (3.17 μg/ml); CM-3269: ITC (16 μg/ml), VRC (4 μg/ml), POS (0.5 μg/ml), FLC (1094.55 μg/ml), AMB (0.2 μg/ml), TRB (2 μg/ml).