Fig. 3.

Accumulation of dietary TG in small intestines of ATGLiKO mice. A–E: Mice fed chow diet were injected with tyloxapol to inhibit peripheral lipolysis. Thereafter they were gavaged with 200 µl corn oil containing 2 µCi [³H]trioleate. A: Radioactivity in the plasma was determined 3 and 6 h after gavage. Mice were euthanized 6 h after gavage, and radioactivity in liver (B) and whole small intestine, duodenum, jejunum, and ileum (C) was measured by scintillation counting. Distribution (D) and relative distribution (E) of radioactivity in lipid species of duodenum 6 h after gavage. Data represent mean values ± SEM (n = 5). *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. F: Radioactivity in the whole small intestine, duodenum, jejunum, and ileum was determined 30 min after gavage of 200 µl corn oil containing 2 µCi [³H]trioleate. Data represent mean values ± SEM (n = 4). *P ≤ 0.05; **P ≤ 0.01. G: Mice were gavaged with 200 µl Evans blue, and gut transit was determined by recording the time until appearance in the feces. Data represent mean values ± SEM (n = 3).