Figure 4.

Both hdc forms work in pruning. ddaC neurons labeled with UAS-CD8-GFP driven by ppk-GAL4. This combination is also used to drive UAS-hdc or UAS-hdcΔ2. A–D, Panel showing that the number of I° and II° dendrites in ddaC neurons in wandering larvae (wL3), before pruning, does not appear to be modified in the different genotypes used for the gain-of-function experiments. E–G, Panel showing that at 18 h APF, ddaC neurons are pruned normally when overexpressing UAS-hdc or UAS-hdcΔ2. H–J, At 18 h APF, the strong severing defect found in hdc⁴³ homozygous background (H) is rescued by the expression of either UAS-hdc (I) or UAS-hdcΔ2 (J). There is more disruption of dendrite clearance in the UAS-hdcΔ2-rescued neurons (J). K, Quantification of both clearance and severing events confirming that both the short and the long forms of HDC can rescue hdc⁴³/hdc⁴³ pruning in a significant manner (p < 0.0001). Nevertheless, the short form (UAS-hdcΔ2) is less efficient than the expression of HDC full length.