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. 2012 Nov 28;32(48):17086–17096. doi: 10.1523/JNEUROSCI.1391-12.2012

Figure 5.

Figure 5.

Role of hdc in other neurons that undergo remodeling. A–C, We used C161-GAL4 to express UAS-CD8::GFP in 5 other da neurons and stain the wL3 fillets with HDC antibody. hdc is expressed in the other class of da neurons, dbd neuron (purple arrows), and ddaC (yellow arrowhead). D–F, We used C161-GAL4 expressing UAS-CD8::GFP to visualize dorsal sensory neurons (D). In wild-type pupae at 24 h APF (E), only two da neurons and dbd neuron remain in the field (arrows), and they have undergone pruning. The three other da neurons labeled in C161-GAL4 have undergo apoptosis by this time. F, In hdc43/hdc43 at 24 h APF, ddaA, B and F are absent, demonstrating that hdc is not required for the apoptosis of da neurons. The remaining neurons, ddaD, ddaE, and dbd, show consistent severing defects (arrows). G–I, Twenty-four hour APF γ-MB neurons labeled with 201Y-Gal4>UAS-mCD8-GFP. G, γ-MB neurons have almost completely pruned both their axons (arrows) and dendrites. H, In MARCM clones for hdc43, the pruning does not appear to be modified, and only a few axons remain (arrows). I, Pruning is suppressed by downregulating EcR with EcR-RNAiCA104, resulting in the persistence of axons at 24 h APF (arrows).