Figure 6.
Regulation of hdc expression. A, MARCM clones for esgG66B show no defects in pruning at 18 h APF, (RedStinger expression in nucleus of homozygous mutant clone), B, C, ppk-GAL4>btd-RNAi (B) and ppk-GAL4>Sp1-RNAi (C) at 18 h APF; the pruning of ddaC neurons is not disrupted in either background. D–F, Fillets of 0 h APF pupa where ddaC neurons are labeled with UAS-CD8-GFP driven by ppk-GAL4, which is also used to drive EcR-RNAiCA104 or Sox14-RNAi when necessary. D, The expression of EcR-RNAiCA104 in ddaC neurons (arrowhead) suppresses hdc expression in a cell autonomous manner. HDC can still be seen in the neighboring da neurons (arrows). E, The expression of Sox14-RNAi in ddaC neurons does not affect hdc expression. F, In a hdc43/hdc43 background, Sox14 staining is still present in the nucleus of ddaC neurons (arrowhead)s.