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ELISA titrations of rabbit anti-spore IgGs specific for the three spore exosporium antigens BclA (▴), ExsFA (▪) and p5303 (•) were carried out on plates coated with wild-type (WT) Ames spores (a) or the BclA deletion mutant of Ames, ΔbclA (b). The negative control (○) was rabbit antibody specific for the unrelated vegetative protein CylI.
