Table 3. Differentially transcribed genes expressed at a higher level in early and late-exponential phase cultures of the S. uberis Vru mutant compared with S. uberis 0140J.
| Gene* | Protein annotation | Early exponential growth phase | Late-exponential growth phase | ||
| Mean fold change† | Mean P value‡ | Mean fold change† | Mean P value‡ | ||
| purH/Sub0030 | Bifunctional phosphoribosyl-aminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase | 91.1/90.0 | <0.001 | 14.8/12.2 | <0.001/0.065 |
| Sub0865 | Guanosine 5′-monophosphate oxidoreductase | 83.1/75.9 | <0.001 | 8.2/7.1 | <0.001 |
| purN/Sub0029 | Phosphoribosylglycinamide formyltransferase | 72.8/63.1 | <0.001 | 10.9/9.1 | <0.001 |
| purF/Sub0027 | Amidophosphoribosyltransferase | 69.8/67.9 | <0.001 | 31.0/29.7 | <0.001 |
| purM/Sub0028 | Phosphoribosylaminoimidazole synthetase | 67.7/65.0 | <0.001 | 21.4/8.6 | 0.033/0.011 |
| Sub0026 | Phosphoribosylformylglycinamidine synthase protein | 60.0/21.5 | <0.001 | 50.7/40.8 | <0.001 |
| purC/Sub0025 | Phosphoribosylaminoimidazole-succinocarboxamide synthase | 20.0/18.0 | <0.001 | 61.7/55.3 | <0.001 |
| purA/Sub0152 | Adenylosuccinate synthetase | 18.9/15.5 | <0.001 | – | – |
| Sub0987 | Hypothetical protein | 14.8/13.6 | <0.001 | – | – |
| purK/Sub0055 | Phosphoribosylaminoimidazole carboxylase ATPase subunit | 12.0/11.3 | <0.001 | 21.2/13.2 | <0.001 |
| purE/Sub0054 | Phosphoribosylaminoimidazole carboxylase catalytic subunit | 8.2/7.6 | <0.001 | 23.4/20.4 | <0.001 |
| purB/Sub0056 | Adenylosuccinate lyase | 7.0/6.5 | <0.001 | 4.9/4.7 | <0.001 |
| purD/Sub0053 | Phosphoribosylamine-glycine ligase | 7.0/6.7 | <0.001 | 18.9/18.0 | <0.001 |
| Sub0410 | Membrane protein | 7.0/6.3 | <0.001 | 4.5/4.3 | <0.001 |
| Sub0411 | PAP2 superfamily protein | 6.1/5.9 | <0.001 | – | – |
| Sub0826 | Surface-anchored subtilase family protein | – | – | 6.6/6.1 | <0.001 |
| fhs1/Sub0942 | Formate-tetrahydrofolate ligase | – | – | 6.1/5.8 | <0.001 |
| spxA/Sub1777 | Transcriptional regulator Spx | 5.9/5.6 | <0.001 | – | – |
| Sub0746 | Membrane protein | 5.4/4.3 | <0.001 | – | – |
| Sub1260 | Formate/nitrite transporter family protein | 4.9/4.4 | <0.001 | – | – |
| fabD/Sub1497 | Malonyl-CoA-acyl carrier protein transacylase | – | – | 4.7/4.7 | <0.001 |
| fabK/Sub1498 | Enoyl-ACP reductase | – | – | 4.4/4.3 | <0.001 |
| Sub1233 | Major facilitator superfamily protein | 4.3/4.2 | <0.001 | – | – |
| rpmB/Sub0331 | 50S ribosomal protein L28 | 4.2/4.2 | 0.01/<0.001 | – | – |
*
Gene and protein annotation according to the genomic sequence of S. uberis 0140J (Ward et al., 2009).
†
Mean fold change represented by S. uberis 0140J versus the S. uberis vru mutant for each of the probes tested.
‡
Where the P value for data from one of the two probes is not in excess of 0.001, the mean of both P values is provided.