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Deletion of the intercellular adhesin locus abrogates biofilm formation in the absence of EGTA. Strains 10833, Newman and their respective ica and clfB single and double deletion mutants were tested for biofilm formation in TSBG (dark grey) and TSBG+12.5 mM EGTA (light grey). Biofilms were stained with safranin, the safranin was solubilized, and A415 was recorded. Results were averaged from three biological replicates, each with four technical replicates. Bars represent mean±sd.
