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Insertional inactivation of the aureolysin gene (aur) augments biofilm formation by Newman in EGTA. Strains 10833 and Newman and their respective aur transposon mutants were tested for biofilm formation in TSBG (dark grey) and TSBG+12.5 mM EGTA (light grey). Safranin-stained biofilms are shown to the right and the quantified A415 results obtained from three biological replicates, each with four technical replicates, are shown on the left. Bars represent mean±sd.
