Fig. 4.

Effect of Ad5–DsRed on NOS activation. (a) Ad5–DsRed infection promotes NO production. BEC cells were infected with Ad5–DsRed for the indicated time and then loaded with DAF-2DA for 2 min. The representative images were acquired using a Leica DM 6000 microscope at the indicated time (in min). (b) eNOS is phosphorylated at S1177 upon Ad5–DsRed infection. BEC cells were mixed with Ad5–DsRed for the indicated time and cell lysates were analysed by immunoblotting with an antibody against phospho-S1177-eNOS. The nitrocellulose membrane was stripped and immunoblotted with an antibody against eNOS to establish equal protein loading.