Fig. 8.

eNOS and dynamin2 are dynamically S-nitrosylated in response to Ad5–DsRed infection. BEC cells were transfected with cDNAs encoding HA-dynamin2 and bovine eNOS for 48 h and then infected with Ad5–DsRed for the indicated time. BEC cell lysates were subjected to a biotin switch assay to detect S-nitrosylated (a) eNOS (SNO-eNOS) or (b) dynamin2 (SNO-Dyn2). Treatment with CysNO served as a positive control and total dynamin2 or eNOS levels are shown to evidence equal protein loading among the different samples.