Table 1.

PBEF and VEGF dual treatment increases the permeability of placental amnion explants, but not reflected. Placental and reflected amnion explants were chronically treated with PBEF (100 ng/ml) and/or acutely exposed to VEGF (20 ng/ml) in parallel with untreated controls. The transfer of 2′,7′-dichlorofluorescein (DCF) through the explants was monitored over 160 min. Best fit regression modeling was performed on the data over the full 160 min of DCF collection and the data for each treatment was defined as either linear or non-linear. Linear regression modeling was then used to analyze the initial 100 min of the data sets for all of the treatment conditions. The respective slopes of each regression line are displayed +/− the standard error.

	Treatment	Best fit regression model of complete 160 minute data set	Timepoint at which all treatments in linear range	Slope of linearized 100 min data set (×10−2)
Placental	No Treatment	Linear	100 min	1.07 ± 0.03
	PBEF-only	Linear	100 min	1.36 ± 0.03
	VEGF-only	Linear	100 min	1.39 ± 0.05b
	PBEF, VEGF	Non-linear	100 min	1.73 ± 0.10a
Reflected	No Treatment	Linear	100 min	0.94 ± 0.04
	PBEF-only	Non-linear	100 min	1.75 ± 0.06a,c
	VEGF-only	Non-linear	100 min	1.51 ± 0.05a
	PBEF, VEGF	Non-linear	100 min	1.19 ± 0.06

^aStatistically significant from “No Treatment” P < 0.005.

^bStatistically significant from “No Treatment” P < 0.05.

^cStatistically significant from “PBEF, VEGF” P < 0.01.