Table 1.

List of the 18 spots identified by LC-ESI-MS/MS whose concentration is modulated by leaf rust infection in barley leaves

Spot ID	Accession number	Species	Protein description	EC	Abb.	Mra/pIa	Mrb/ pIb	Cov. (%) c
Photosynthesis and energy metabolism
2577	Q40073	Hordeum vulgare	Ribulose bisphosphate carboxylase/oxygenase activase A, chloroplastic		RuACS (b)	40.2 / 5.3	46.1 / 5.6 e	43.5 e
2581	Q40073	Hordeum vulgare	Ribulose bisphosphate carboxylase/oxygenase activase A, chloroplastic		RuACS (c)	40.8 / 5.2	46.1 / 5.6 e	34.6 e
2760	P05698	Hordeum vulgare	Ribulose bisphosphate carboxylase large chain	4.1.1.39	RuLC	25.4 / 6.4	52.9 / 6.2 e	16.3 e
2776	P05698	Hordeum vulgare	Ribulose bisphosphate carboxylase large chain	4.1.1.39	RuLC	24.2 / 6.4	52.9 / 6.2 e	22.6 e
2866	P05698	Hordeum vulgare	Ribulose bisphosphate carboxylase large chain	4.1.1.39	RuLC	22.3 / 5.2	52.9 / 6.2 e	8.0 e
2952	P05698	Hordeum vulgare	Ribulose bisphosphate carboxylase large chain	4.1.1.39	RuLC	21.3 / 5.1	52.9 / 6.2 e	9.0 e
2983	P05698	Hordeum vulgare	Ribulose bisphosphate carboxylase large chain	4.1.1.39	RuLC	20.1 / 5.3	52.9 / 6.2 e	4.6 e
3004	XP_002465461	Sorghum bicolor	ATP synthase β chaind		ATPase β	19.4 / 4.1	22.8 / 5.3	6.1
3009	P05698	Hordeum vulgare	Ribulose bisphosphate carboxylase large chain	4.1.1.39	RuLC	19.2 / 5.2	52.9 / 6.2 e	4.2 e
3118	P05698	Hordeum vulgare	Ribulose bisphosphate carboxylase large chain	4.1.1.39	RuLC	19.7 / 5.0	52.9 / 6.2 e	6.9 e
3142	P05698	Hordeum vulgare	Ribulose bisphosphate carboxylase large chain	4.1.1.39	RuLC	20.4 / 4.7	52.9 / 6.2 e	4.4 e
3150	P05698	Hordeum vulgare	Ribulose bisphosphate carboxylase large chain	4.1.1.39	RuLC	20.7 / 4.8	52.9 / 6.2 e	4.8 e
Carbohydrate metabolism
2333	CAZ64535	Hordeum vulgare	Sucrose synthase	2.4.1.13	SuSy	88.2 /5.9	92.2 / 5.8	18.4
2962	CAC32847	Hordeum vulgare	Adenosine diphosphate glucose pyrophosphatase		AGPPase	21.0 / 5.1	19.5 / 5.7 e	17.4 e
Protein degradation
2449	XP_002454700	Sorghum bicolor	Leucine aminopeptidase 2,chloroplasticd	3.4.11.1	LAP 2	57.0 / 5.6	61.8 / 7.6	20.0
Defence responses
2886	CAA55345	Hordeum vulgare	chitinase	3.2.1.14	CHI	22.3 / 5.2	26.6 / 6.1	29.4
2956	BAD31057	Oryza sativa	Chitinase III-like protein		CHI III	21.2 / 5.3	18.9 / 6.5	12.7
Secondary metabolism
2613	CAA54616	Hordeum vulgare	Flavonoid 7-O-methyltransferase	2.1.1.6	F-OMT	39.2 / 5.4	42.3 / 5.4	29.5

Statistical information about LC-ESI-MS/MS analysis are reported in Additional files 1 and 2.

Abb.: abbreviation.

a: experimental molecular weight and pI.

b: theoretical molecular weight and pI.

c: amino acid coverage (%).

d: annotation obtained by BLAST-P alignment analysis against the Viridiplantae subset of the nr-database at NCBI.

e: value referred to the mature form of the protein.