FIGURE 2:

Starvation can induce desiccation tolerance. (A) Exponential-phase BY4742 cells were transferred from rich medium containing glucose to the same medium without glucose, and samples were taken at the indicated times and assayed for desiccation tolerance. (B) Strain DBY12000 was grown under defined media conditions in a chemostat at constant doubling time at the times and nutrient-limiting conditions indicated until steady-state conditions were reached, and then were tested for desiccation tolerance using the described protocol (see Materials and Methods). The experiment was performed in triplicate, and representative results are shown.