FIGURE 7:

The Ras-cAMP pathway modulates desiccation tolerance. (A) Model depicting Ras-cAMP regulation of desiccation tolerance. (B) Exponential-phase cultures of the indicated genotype grown in rich medium were assayed for desiccation tolerance. (C) Exponential-phase ras2Δ cells carrying either a vector expressing a dominant-negative allele of RAS2 or an empty vector were grown in SC medium lacking leucine and methionine for 24 h then assayed for desiccation tolerance. (D) Exponential-phase cells carrying a vector expressing PDE2, MSI1, or an empty-vector control were grown in yeast extract peptone (YEP) with 2% galactose for 24 h then assayed for desiccation tolerance. Exponential-phase cells carrying a vector expressing BCY1 or an empty-vector control were grown in SC medium lacking histidine and assayed for desiccation tolerance. (E) Cells with either cyr1^ts allele or the wild-type allele of CYR1 were grown to exponential phase at 23°C and then assayed for desiccation tolerance or transferred to the same medium at 30°C for 6 h and then assayed for desiccation tolerance.