FIGURE 9:

Decreases in ribosome biogenesis can increase desiccation tolerance. (A) Wild-type cells grown in rich medium were transferred to the same medium containing the indicated concentrations of cycloheximide (CHX) and were assayed for desiccation tolerance at the indicated time points. (B) Strains containing a temperature-sensitive allele of a ribosome biogenesis gene or the wild-type strain were grown in rich medium to exponential phase at 23°C, shifted to 33°C for 6 h, then assayed for desiccation tolerance and growth rate. Presented here is only the desiccation tolerance. (C) Here the same data for desiccation tolerance are shown as in (B), but they are now plotted against the doubling time. The wild-type strain has a striped box, whereas all mutant strains have a solid black box. A logarithmic-regression line was fitted to these data. The equation for this line is y = 0.0093e–0.337x, and the R² value of this line is displayed.