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Purification summary for laccase produced by P. ostreatus IBL-02 during decolorization of DBR K- 4BL
| Purification steps | Total volume (mL) | Total enzyme activity (U) | Total protein content (mg) | Specific activity (U/mg) | % Yield | Purification fold |
|---|---|---|---|---|---|---|
| Crude Enzyme |
500 |
62500 |
2360 |
134.95 |
1 |
100 |
| (NH4)2SO4 |
30 |
48060 |
138 |
348.26 |
2.5 |
76 |
| Precipitation | ||||||
| DEAE-Sepharose |
12 |
9440 |
20.34 |
464.11 |
3.4 |
15 |
| Sephadex-G-100 | 09 | 7020 | 12.24 | 573.52 | 4.2 | 11 |
