. 2012 Dec 13;4:21. doi: 10.1186/1757-4749-4-21

Table 3.

Virulence genes, primers and DNA target sequence sizes (amplicons) of multiplex PCRs

Gene	Primers (5^′- 3>^′)	Amplicon (bp)	References
stx	F- GAGCGAAATAATTTATATGTG	518	[17]
stx	R- TGATGATGGCAATTCAGTAT	518	[17]
eae	F- CCCGAATTCGGCACAAGCATAAGC	881	[17]
eae	R- CCCGGATCCGTCTCGCCAGTATTCG	881	[17]
sigA	F- CCGACTTCTCACTTTCTCCCG	430	[20]
sigA	R- CCATCCAGCTGCATAGTGTTTG	430	[20]
sepA	F- GCAGTGGAAATATGATGCGGC	794	[20]
sepA	R- TTGTTCAGATCGGAGAAGAACG	794	[20]
pic	F- ACTGGATCTTAAGGCTCAGGAT	572	[20]
pic	R- GACTTAATGTCACTGTTCAGCG	572	[20]
aggR	F- GTATACACAAAAGAAGGAAGC	254	[17]
aggR	R- ACAGAATCGTCAGCATCAGC	254	[17]
ipaH	F- GTTCCTTGACCGCCTTTCCGATACCGTC	619	[17]
ipaH	R- GCCGGTCAGCCACCCTCTGAGAGTAC	619	[17]
elt	F- TCTCTATGTGCATACGGAGC	322	[17]
elt	R- CCATACTGATTGCCGCAAT	322	[17]
pet	F- GGCACAGAATAAAGGGGTGTTT	302	[20]
pet	R- CCTCTTGTTTCCACGACATAC	302	[20]
sat	F-TCAGAAGCTCAGCGAATCATTG	930	[20]
sat	R-CCATTATCACCAGTAAAACGCACC	930	[20]