Fig. 3.
Stress-inducible expression of OsSRO1c. (A) Expression of OsSRO1c under various abiotic stresses and ABA treatments. Four-leaf stage seedlings were subjected to drought stress (grow without water supply), salt (200mM NaCl), cold (4 ºC), heat (42 ºC), UV, wounding, H2O2 (1%), and ABA (100 µM). Relative expression levels of OsSRO1c were examined by real-time quantitative RT–PCR at the indicated time points. Error bars indicate the SE based on three replicates. (B) Expression pattern of GFP driven by the OsSRO1c promoter in transgenic rice plants under normal conditions (Ba–Bh) or dehydration stress (Bi–Bp). Shown are root (Ba and Bi), leaves (Bb and Bj), leaf sheath (Bc and Bk), ligule and auricle (Bd and Bl), calli (Be and Bm), stem and nodes (Bf and Bn), stamen and pistil (Bg and Bo), and hull (Bh and Bp). The scale bar indicates 2mm. (C) Optical (Ca and Cb) and confocal (Cc) microscopic analyses of GFP induction in guard cells by dehydration. The leaves of POsSRO1c: GFP transgenic rice were observed after dehydration stress for 5h.