Table 1.

Description of the 12 SSR markers used to assess the genome integrity of S. bulbocastanum and S. commersonii synthetic tetraploids and the diploid parents (cmm1t and blb1C) they were derived from. For each marker, the respective name, source, repeat motifs, forward (Fw) and reverse (Rv) primer sequences, annealing temperature (T _a), map location, and amplified allele sizes are reported.

Name	Repeat motif	Primer sequences	T a (C°)	Map location	Allele size (nt)a
STG0001	(CT)n	Fw_CAGCCAACATTTGTACCCCT	55	XI	C: 126, 158
		Rv_ACCCCCACTTGCCATATTTT			B: 130, 138
STI004	(AAG)n	Fw_GCTGCTAAACACTCAAGCAGAA	57	VI	C: 66, 72
		Rv_CAACTACAAGATTCCATCCACAG			B: 78, 81
STI0012	(ATT)n	Fw_GAAGCGACTTCCAAAATCAGA	53	IV	C: 179, 188
		Rv_AAAGGGAGGAATAGAAACCAAAA			B: 167
STI0030	(ATT)n	Fw_TGACCCTCCAACTATAGATTCTTC	56	XII	C: 133
		Rv_TGACAACTTTAAAGCATATGTCAGC			B: 79
STI0032	(GGA)n	Fw_TGGGAAGAATCCTGAAATGG	54	V	C: 118
		Rv_TGCTCTACCAATTAACGGCA			B: 115, 118
STM0031	(AC)n…(AC)n GCAC (AC)n (GCAC)n	Fw_CATACGCACGCACGTACAC	52	VII	C: 63
		Rv_TTCAACCTATCATTTTGTGAGTCG			B: ND
STM1052	(AT)n GT (AT)n (GT)n	Fw_CAATTTCGTTTTTTCATGTGACAC	54	IX	C: ND
		Rv_ATGGCGTAATTTGATTTAATACGTAA			B: 207, 224
STM1053	(TA)n (ATC)n	Fw_TCTCCCCATCTTAATGTTTC	55	III	C: 120, 172
		Rv_CAACACAGCATACAGATCATC			B: 120, 172
STM1104	(TCT)n	Fw_TGATTCTCTTGCCTACTGTAATCG	57	VIII	C: 195
		Rv_CAAAGTGGTGTGAAGCTGTGA			B: 165, 177
STM1106	(ATT)n	Fw_TCCAGCTGATTGGTTAGGTTG	56	X	C: 174
		Rv_ATGCGAATCTACTCGTCATGG			B: 141, 150
STM5114	(ACC)n	Fw_AATGGCTCTCTCTGTATGCT	53	II	C: 289
		Rv_GCTGTCCCAACTATCTTTGA			B: 286
STM5127	(TCT)n	Fw_TTCAAGAATAGGCAAAACCA	55	I	C: 246
		Rv_CTTTTTCTGACTGAGTTGCCTC			B: 243

^a C, S. commersonii; B, S. bulbocastanum; ND, not detected.