Table 2.
Results from MSAP fingerprinting analysis carried out on diploid S. commersonii (cmm1t) and S. bulbocastanum (blb1c) progenitors and their related autotetraploids. Values refer to the total number of scored fragments and the genuine epigenetic polymorphic markers (called unambiguous, see Results), and the number (%) of hyper- and hypomethylation events at CG and CHG sites occurring in each tetraploid with respect to its diploid progenitor. The methylation ratio (MR) is also shown.
| Genotype | Scored, n | Unambiguous, n | Hyper, n (%) | Hypo, n (%) | MRa | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| CG | CHG | CG | CHG | ||||||||
| S. commersonii | |||||||||||
| cmm15 | 749 | 460 | 24 | (5.22) | 20 | (4.35) | 16 | (3.48) | 13 | (2.83) | 1.52 |
| cmm23 | 837 | 594 | 22 | (3.70) | 16 | (2.69) | 15 | (2.53) | 26 | (4.38) | 0.93 |
| cmm24 | 720 | 494 | 14 | (2.83) | 22 | (4.45) | 15 | (3.04) | 20 | (4.05) | 1.03 |
| cmm27 | 789 | 518 | 14 | (2.70) | 21 | (4.05) | 7 | (1.35) | 10 | (1.93) | 2.06 |
| cmm30 | 789 | 479 | 15 | (3.13) | 25 | (5.22) | 10 | (2.09) | 17 | (3.55) | 1.48 |
| Average | 776.80 | 509.00 | 17.80 | (3.50) | 20.80 | (4.13) | 12.60 | (2.48) | 17.20 | (3.32) | 1.32 |
| S. bulbocastanum | |||||||||||
| blb10 | 366 | 215 | 5 | (2.33) | 2 | (0.93) | 4 | (1.86) | 2 | (0.93) | 1.17 |
| blb22 | 455 | 324 | 15 | (4.63) | 3 | (0.93) | 5 | (1.54) | 3 | (0.93) | 2.25 |
| blb25 | 389 | 212 | 2 | (0.94) | 2 | (0.94) | 3 | (1.42) | 3 | (1.42) | 0.66 |
| blb26 | 451 | 283 | 4 | (1.41) | 4 | (1.41) | 8 | (2.83) | 4 | (1.41) | 0.67 |
| Average | 415.25 | 258.50 | 6.50 | (2.33) | 2.75 | (1.05) | 5.00 | (1.91) | 3.00 | (1.17) | 1.10 |
a Ratio between hyper- and hypomethylation frequencies (CG hyper+CHG hyper)/(CG hypo+CHG hypo).