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. 2013 Jan 31;64(2):675–684. doi: 10.1093/jxb/ers361

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© 2013 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — Overexpression of ABI5 altered flowering time. (A) The expression level of ABI5-HA fusion protein in individual transgenic lines of p35S::ABI5-HA. (B) Flowering-time phenotypes in abi5-4 (Ws), ABI5-5 (Col) and ABI5-18 (Col) under long-day conditions. (C and D) Comparisons of total rosette leaf numbers (n > 15 for each experiment) and FLC expression under long-day (LD) (C) and short-day (SD) conditions (D) (*P < 0.05). (E) FLC expression in FLC abi5-4 transgenic lines (T2, homozygous) (#2, #3, #5, and #6 are individual lines). Total RNA was extracted from 10-day-old seedlings. ACTIN2 represents the loading control. (F) Comparisons of total rosette leaf numbers. Data are mean ± standard errors of three replicated experiments (n > 15 for each experiment).