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. 2013 Jan 31;64(2):675–684. doi: 10.1093/jxb/ers361

Fig. 6.

Fig. 6.

ABI5 directly binds to DNA elements in the FLC promoter. (A) Schematic diagram showing the fusion of reporter constructs of FLC::LUC; triangles indicate the positions of putative ABRE motifs (PyACGTGG/TG) located in the 2.8-kb FLC promoter segment; arrows indicate mutated sequences of ABRE-like and G-box motifs. (B) Schematic diagram to show FLC promoter elements; DNA1–6 indicate the DNA fragments; pairs of arrows represent the DNA fragments amplified in the chromatin immunoprecipitation assay. (C) Chromatin immunoprecipitation enrichment to show the binding ability of ABI5-HA to the DNA fragments of the FLC promoter, as shown by qRT-PCR. InPut, total input chromatin DNA. Data are mean ± standard error of one experiment (n > 3). Similar results were obtained from four independent experiments. (D) Mutated ABRE-like elements in the FLC promoter could partially abolish FLC promoter activity in a transient expression assay. Data are mean ± standard errors of three replicated experiments (n > 3 for each experiment).