Fig. 5.

Purified egg lipids activate PR-1 gene expression. Plants were treated with 2 µl of P. brassicae egg extract, total egg lipids, and a fraction eluting at 100% MeOH from a solid-phase extraction column (SPE-F) for 24h. Untreated plants (CTL) and plants treated with 2 µl of water or 10% DMSO were used as controls. The relative expression level of PR-1 was analysed by quantitative PCR. Expression levels were normalized with respect to the housekeeping gene EIF4A. Data bars represent the mean (±SE) of three technical repeats. The experiment was repeated once with similar results.