Fig. 6.

Involvement of an L-type lectin receptor kinase in egg perception. (A) Gene structure of LecRK-I.8 (At5g60280) showing the T-DNA insertion site of the mutant studied here. The positions of primers used for RT-PCR are shown. (B) Analysis of LecRK-I.8 expression in Col-0 and in the T-DNA insertion line lecrk-I.8 by RT-PCR. EIF4A was used as a control. Plants were treated with 2 µl of P. brassicae egg extract for 72h (+). Untreated plants were used as controls (–). (C) The relative expression level of PR-1 was analysed by quantitative PCR. Leaves from Col-0 and lecrk-I.8 were treated with 2 µl of P. brassicae egg extract for 24, 48, and 72h before RNA extraction. Expression levels were normalized with respect to the housekeeping gene EIF4A. Data bars represent the mean (±SE) of three technical repeats. This experiment was repeated once with similar results.