Table 2.
Experimental validation of a subset of cytokinin/auxin-regulated transcripts. Shown are the log2 fold-change values from qPCR versus RNA sequencing in root tip (RT), lateral root (LR), and whole root (WR) for genes induced by cytokinin (A) or auxin (B) and repressed by cytokinin and auxin (C).
| Gene ID | Description | Tissue | log2 fold-change | |
|---|---|---|---|---|
| mRNA sequencing | qPCR | |||
| (A) Genes induced by cytokinin | ||||
| Solyc04g078900 | Cytochrome P450 | RT | 4.67 | 4.71 |
| LR | 5.66 | 3.56 | ||
| WR | 7.00 | 2.65 | ||
| Solyc12g008900 | SlCKX6 | RT | 5.39 | 4.37 |
| LR | 7.30 | 3.67 | ||
| WR | 7.31 | 3.39 | ||
| Solyc01g111900 | O-Methyltransferase | RT | 6.69 | 5.54 |
| LR | 4.78 | 4.88 | ||
| WR | 4.12 | 3.83 | ||
| Solyc01g090810 | Expansin | RT | 3.88 | 4.22 |
| LR | 3.76 | 3.12 | ||
| WR | 5.97 | 5.51 | ||
| Solyc12g100150 | LOB domain | RT | 4.34 | 4.61 |
| LR | 4.84 | 4.28 | ||
| WR | 5.98 | 3.99 | ||
| (B) Genes induced by auxin | ||||
| Solyc01g090810 | Expansin | RT | 7.27 | 6.54 |
| LR | 7.32 | 6.39 | ||
| WR | 5.16 | 4.74 | ||
| Solyc03g120380 | SlIAA11 | RT | 4.91 | 4.76 |
| LR | 5.68 | 5.08 | ||
| WR | 5.60 | 4.65 | ||
| Solyc07g008020 | SlIAA19 | RT | 7.58 | 7.41 |
| LR | 6.92 | 7.62 | ||
| WR | 7.16 | 6.87 | ||
| Solyc03g120060 | Cytochrome P450 | RT | 7.85 | 7.61 |
| LR | 6.80 | 7.65 | ||
| WR | 5.70 | 5.68 | ||
| Solyc02g064830 | SlGH3-3 | RT | 7.30 | 8.11 |
| LR | 6.60 | 8.06 | ||
| WR | 8.22 | 6.88 | ||