. 2012 Dec 15;11:160. doi: 10.1186/1475-2859-11-160

Table 1.

Strains and plasmids used in this study

Strain	Genotype / Description	Source
L. lactis htrA NZ9000	Mutant MG1363 derivative (nisRK genes on the chromosome) lacking htrA	[68]
E. coli TG1	supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5 (rK– mK–) [F′ traD36 proAB lacIqZΔM15]	ATCC
E. coli BL21 (DE3)	F^–ompT gal dcm lon hsdS_B(r_B^-m_B^-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])	Novagen
Plasmid
pET28(b)	Km^r	Novagen
pAW528	Cm^r, Amp^r; pBS::pIL252::t_trpA::P_nisA::rbs_nisA::sp_Usp45- CBD-coh1C3-cwa_M6-tlt2	[42]
pAW531	Cm^r, Amp^r; pBS::pIL252::t_trpA::P_nisA::rbs_nisA::sp_Usp45-CBD-cwa_M6-tlt2	[42]
pAW549	Cm^r, Amp^r; pBS::pIL252::t_trpA::P_nisA::rbs_nisA::sp_Usp45-CBD-coh2O2-cwa_M6-tlt2	This Work
pAW564	Cm^r, Amp^r; pBS::pIL252::t_trpA::P_nisA::rbs_nisA::sp_Usp45-CBD-Lk-coh2O2-cwa_M6-tlt2	This Work
pAW596	Cm^r, Amp^r; pBS::pIL252::t_trpA::P_nisA::rbs_nisA::sp_Usp45-CBD-coh2O2-Lk-cwa_M6-tlt2	This Work
pAW594	Cm^r, Amp^r; pBS::pIL252::t_trpA::P_nisA::rbs_nisA::sp_Usp45-CBD-Lk-coh2O2-Lk-cwa_M6-tlt2	This Work
pAW546	Cm^r, Amp^r; pBS::pIL252::t_trpA::P_nisA::rbs_nisA::sp_Usp45-CBD-coh1C3-coh2O2-cwa_M6-tlt2	This Work
pAW561	Cm^r, Amp^r; pBS::pIL252::t_trpA::P_nisA::rbs_nisA::sp_Usp45-CBD-coh1C3-Lk-coh2O2-cwa_M6-tlt2	This Work
pAW595	Cm^r, Amp^r; pBS::pIL252::t_trpA::P_nisA::rbs_nisA::sp_Usp45-CBD-coh1C3-coh2O2-Lk-cwa_M6-tlt2	This Work
pAW592	Cm^r, Amp^r; pBS::pIL252::t_trpA::P_nisA::rbs_nisA::sp_Usp45-CBD-coh1C3-Lk-coh2O2-Lk-cwa_M6-tlt2	This Work
pAW579	Cm^r, Amp^r; pBS::pIL252::t_trpA::P_nisA::rbs_nisA::sp_Usp45-CBD-coh2S1-cwa_M6-tlt2	This Work
pAW576	Cm^r, Amp^r; pBS::pIL252::t_trpA::P_nisA::rbs_nisA::sp_Usp45-CBD-coh1C3-coh2S1-cwa_M6-tlt2	This Work
pETdock1	Kn^r; pET28(b)::with cloned dock1 from celS	[42]
pETdock2	Kn^r; pET28(b)::with cloned dock2 from cipA	This Work
pETUdock1	Kn^r; pET28(b)::PT7::6xHis-uidA-dock1	[42]
pETUdock2	Kn^r; pET28(b)::PT7::6xHis-uidA-dock2	This Work
pETU	Kn^r; pET28(b)::PT7::6xHis-uidA	[42]
pETLdock1	Kn^r; pET28(b)::PT7::6xHis-lacZ-dock1	This Work
pETLdock2	Kn^r; pET28(b)::PT7::6xHis-lacZ-dock2	This Work
pETL	Kn^r; pET28(b)::PT7::6xHis-lacZ	This Work

The pAW500 series of vectors are designed for the cell-wall targeting of various scaffold protein permutations consisting of cohesins from CipA and OlpB or SdbA. coh1C3, type 1 cohesin of CipA; coh2O2, type 2 cohesin of OlpB; coh2S1, type 2 cohesin of SdbA; PT7, inducible T7 promoter; P_nisA, inducible nisA promoter; rbs_usp45, Usp45 ribosome-binding site; rbs_nisA, nisA ribosome-binding site; sp_Usp45, signal sequence of Usp45; cwa_M6, anchor motif of M6 protein; tlt2, transcriptional terminator of rrnB operon; t_trpA, transcriptional terminator of trpA; Lk, olpB linker region.