Fig. 4.

RISC complexes from RSV 6340WT-infected cells are enriched for CCND1 and let-7f transcripts. (a) RISC-associated RNA from mock-, 6340WT- and 6340ΔG-infected cells were assayed for CCND1 by PCR. CCND1 UTR amplicons (0.6 kb) were amplified as described in Methods in two independent experiments. 18S rRNA was used as a loading control. RAgo, Anti-Ago2-precipitated RNA from RSV-infected cells; RBU, anti-BrdU-precipitated RNA from RSV-infected cells; VAgo, mock-infected Vero cell RNA precipitated with anti-Ago2; VBU, mock-infected Vero cell RNA precipitated anti-BrdU. (b) Enrichment of let-7f in RISC immunoprecipitated RNA from 6340WT- and RSVΔG-infected cells was assayed by qPCR and normalized to that of mock-infected cells from two independent experiments. Results are shown as means±sem, and Student’s t-test was used to measure the statistical significance of the data: ***P<0.001.