Table 1.
Identification of the best pairs of genes for different treatments, species, and tissues
|
A | |||||||
|---|---|---|---|---|---|---|---|
| Species | Software | CTRL | DRT | REC | CTRL+DRT+REC Apex | CTRL+DRT+REC Base | All treatments |
|
E. globulus |
GeNorm |
A-Tub/UBQ |
A-Tub/H2B |
IDH/SAND |
UBQ/H2B |
H2B/A-Tub |
H2B/UBQ |
| NormFinder |
IDH/ACT |
ACT/A-Tub |
A-Tub/IDH |
IDH/A-Tub |
A-Tub/ACT |
A-Tub/ACT |
|
|
E. urograndis |
GeNorm |
SAND/A-Tub |
H2B/UBQ |
A-Tub/SAND |
UBQ/A-Tub |
IDH/ACT |
IDH/ACT |
| NormFinder |
ACT/SAND |
UBQ/H2B |
SAND/A-Tub |
ACT/SAND |
A-Tub/SAND |
ACT/SAND |
|
|
E. uroglobulus |
GeNorm |
H2B/UBQ |
EF1/IDH |
IDH/ACT |
SAND/IDH |
IDH/18S |
A-Tub/ACT |
| NormFinder |
A-Tub/EF1 |
SAND/ACT |
18S/H2B |
H2B/A-Tub |
A-Tub/ACT |
A-Tub/H2B |
|
|
All species |
GeNorm |
UBQ/H2B |
IDH/SAND |
IDH/ACT |
IDH/ACT |
IDH/ACT |
IDH/ACT |
| |
NormFinder |
IDH/SAND |
ACT/IDH |
SAND/IDH |
SAND/ACT |
SAND/IDH |
SAND/IDH |
|
B | |||||||
|
Species |
Software |
CTRL |
COLD |
CTRL+COLD Apex |
CTRL+COLD Base |
All treatments |
|
|
E. globulus |
GeNorm |
A-Tub/UBQ |
EF1/IDH |
EF1/IDH |
SAND/EF1 |
EF1/IDH |
|
| NormFinder |
ACT/UBQ |
SAND/UBQ |
ACT/A-Tub |
ACT/SAND |
ACT/A-Tub |
|
|
|
E. urograndis |
GeNorm |
IDH/UBQ |
A-Tub/UBQ |
UBQ/A-Tub |
UBQ/18S |
A-Tub/UBQ |
|
| NormFinder |
IDH/UBQ |
SAND/UBQ |
SAND/IDH |
ACT/IDH |
IDH/SAND |
|
|
|
E. uroglobulus |
GeNorm |
IDH/ACT |
UBQ/SAND |
SAND/UBQ |
B-tub/SAND |
UBQ/SAND |
|
| NormFinder |
A-Tub/H2B |
ACT/IDH |
H2B/UBQ |
A-Tub/ACT |
H2B/A-Tub |
|
|
|
All species |
GeNorm |
SAND/UBQ |
ACT/SAND |
UBQ/IDH |
IDH/SAND |
IDH/SAND |
|
| |
NormFinder |
A-Tub/EF1 |
IDH/ACT |
H2B/UBQ |
IDH/SAND |
IDH/SAND |
|
|
C | |||||||
|
Species |
Software |
DRT+COLD Apex |
DRT+COLD Base |
All treatments |
|
|
|
|
E. globulus |
GeNorm |
IDH/A-Tub |
IDH/SAND |
IDH/ACT |
|
|
|
| NormFinder |
UBQ/IDH |
UBQ/A-Tub |
UBQ/A-Tub |
|
|
|
|
|
E. urograndis |
GeNorm |
H2B/UBQ |
SAND/A-Tub |
IDH/ACT |
|
|
|
| NormFinder |
ACT/A-Tub |
SAND/A-Tub |
ACT/IDH |
|
|
|
|
|
E. uroglobulus |
GeNorm |
IDH/ACT |
EF1/IDH |
IDH/ACT |
|
|
|
| NormFinder |
H2B/A-Tub |
A-Tub/ACT |
A-Tub/H2B |
|
|
|
|
|
All species |
GeNorm |
ACT/IDH |
IDH/SAND |
IDH/ACT |
|
|
|
| |
NormFinder |
ACT/IDH |
SAND/IDH |
IDH/ACT |
|
|
|
|
D | |||||||
|
Species |
Software |
in vitro (dark and light) |
Greenhouse (dark and night) |
All treatments |
|
|
|
|
E. globulus |
GeNorm |
H2B/IDH |
EF1/ACT |
UBQ/IDH |
|
|
|
| NormFinder | UBQ/ACT | IDH/EF1 | ACT/IDH | ||||
A – Water stress experiment; B – Low-temperature experiment; C – Water stress + low-temperature experiments in tissues (apex and base), D – light experiments. CTRL = control; DRT – water stress; REC – recovery from water stress; COLD – low temperature.