Table 3.
Gene identification and primers sequences used in the qPCR analyses
| Gene | Primer | Primer sequence (5’-3’) | Amplicon (bp)* | Amplification efficiency (%)** |
|---|---|---|---|---|
| Elongation factor-1α |
Forward |
CCTGTCCTTGATTGTCACACTTCC |
130 |
110 |
| Reverse |
CCATTCCAGCATCACCGTTCTTC |
|||
| Ubiquitin (E. gobulus) |
Forward |
TCCGTCAAAAGCGAACAGA |
173 |
97 |
| Reverse |
CATTTCCCTCCAGATTACCC |
|||
| Ubiquitin (E. urograndis) |
Forward |
GGACTTTCGTTCGTTTTGGT |
107 |
97 |
| Reverse |
GTGATTTGGGGAGGGTTTG |
|||
| Actin |
Forward |
AGATGACCCAGATTATGTTTGAGACCTTC |
122 |
97 |
| Reverse |
ACCATCACCAGAATCCAACACAATACC |
|||
| SAND protein |
Forward |
TGGGTCACACAGGATTTTGA |
130 |
100 |
| Reverse |
CTCCCAGCAAAAAGATCTCG |
|||
| Isocitrate dehydrogenase-NADP |
Forward |
AGTTTGAGGCTGCTGGAATC |
100 |
103 |
| Reverse |
CTTGCATGCCCACACATAAC |
|||
| Histone H2B |
Forward |
AACAAGAAGCCCACCATCAC |
142 |
96 |
| Reverse |
ACAACTTCCTCCTCGCTCAC |
|||
| α-Tubulin |
Forward |
CCAGTGAACAAATGCCCTCT |
92 |
107 |
| Reverse |
TGATCAGCAACAACACAGCA |
|||
| Ribossomal 18S |
Forward |
CATGGCCGTTCTTAGTTGGT |
71 |
95 |
| Reverse |
TAGCAGGCTGAGGTCTCGTT |
|||
| β-Tubulin |
Forward |
GATGGGGACGCTATTGATTT |
225 |
100 |
| Reverse | CTTGGGTTGATGAGTTTCAGG |
* Melting temperature = 60°C for all primers. **E=10^(-1/slope)-1.