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. 2012 Nov 7;7:187. doi: 10.1186/1748-717X-7-187

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Copyright ©2012 Lindel et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Plot of flow cytometry using intracellular staining: No change of pRB labeling in W12 cells (intact E2-gene) after irradiation. Legend 5: A = control group, B = 2 Gy, C = 7 Gy: In each case negative control were cells treated as described above without Rb-ak staining. Isotypic control were cells treated with isotype–matched control of irrelevant specificity from FITC Mouse IgG1 Isotype control (BD-Set# 556538) instead of Rb-ak staining or p53-ak staining. Analyses were performed after 0 h and 24 h irradiation with 2 Gy and 7 Gy.W12 cells (intact E2-gene) showed 80%, 77% and 80% pRb-labeled cells after irradiation with 0 Gy, 2 Gy and 7 Gy, respectively.