Table 3.
Distribution of the SSR frequency in Brassica EST sequences
| Motifs | Number | Percentage (%) | Total length (bp) | Average length (bp) |
|---|---|---|---|---|
| Mononucletide |
339538 |
76.277 |
7614099 |
22.42 |
| A |
310831 |
69.828 |
7256120 |
23.34 |
| C |
28707 |
6.449 |
357979 |
12.47 |
| Dinucleotide |
55734 |
12.521 |
935952 |
16.79 |
| AG |
44403 |
9.975 |
756440 |
17.04 |
| AT |
6090 |
1.368 |
99192 |
16.29 |
| AC |
5186 |
1.165 |
79618 |
15.35 |
| CG |
55 |
0.012 |
702 |
12.76 |
| Trinucleotide |
48202 |
10.829 |
818475 |
16.98 |
| AAG |
17256 |
3.877 |
298896 |
17.32 |
| AGG |
7389 |
1.660 |
123783 |
16.75 |
| ACT |
5199 |
1.168 |
88563 |
17.03 |
| ACC |
3878 |
0.871 |
62421 |
16.10 |
| AAC |
3644 |
0.819 |
61962 |
17.00 |
| AGT |
3433 |
0.771 |
58869 |
17.15 |
| AGC |
2305 |
0.518 |
38754 |
16.81 |
| ACG |
2063 |
0.463 |
33597 |
16.29 |
| CCG |
1558 |
0.350 |
25122 |
16.12 |
| AAT |
1477 |
0.332 |
26508 |
17.95 |
| Tetranucleotide |
1026 |
0.230 |
23372 |
22.78 |
| AAAG |
249 |
0.056 |
5784 |
23.23 |
| AAAC |
164 |
0.037 |
3484 |
21.24 |
| AAGG |
84 |
0.019 |
1912 |
22.76 |
| AACG |
82 |
0.018 |
1844 |
22.49 |
| Others |
447 |
0.100 |
10348 |
23.15 |
| Pentanucleotide |
220 |
0.049 |
5980 |
27.18 |
| AAACC |
40 |
0.009 |
1195 |
29.88 |
| AAAAG |
36 |
0.008 |
935 |
25.97 |
| AGAGG |
21 |
0.005 |
570 |
27.14 |
| AAAAC |
20 |
0.004 |
515 |
25.75 |
| Others |
103 |
0.023 |
2765 |
26.84 |
| Hexanucleotide |
419 |
0.094 |
13704 |
32.71 |
| AAAAAC |
42 |
0.009 |
1326 |
31.57 |
| ACCTGC |
29 |
0.007 |
870 |
30.00 |
| AAGGTG |
23 |
0.005 |
690 |
30.00 |
| AGGAGT |
19 |
0.004 |
570 |
30.00 |
| Others |
306 |
0.069 |
10248 |
33.49 |
| Total | 445139 | 100 | 9411582 | 21.14 |