Figure 6. The glucokinase double mutant is hypersusceptible to hydrogen peroxide, but attenuated in phagocyte oxidase deficient mice.

(A) Susceptibility to low pH, reactive nitrogen intermediates and hydrogen peroxide. Strains were exposed to pH 4.5 for 6 days, to 3 mM NaNO₂ at pH 5.5 for 3 days and to 5 mM H₂O₂ for 4 hrs and bacterial survival was determined by plating CFU. Data are means ± s.d. of triplicate cultures. Hypersusceptibility of ΔppgKΔglkA to 5 mM H₂O₂ was demonstrated in three independent experiments. (B) Bacterial titers in lungs of gp96^phox−/− mice infected with wt and ΔppgKΔglkA. Data are means ± s.d. from three mice per time point per group and representative of two independent experiments.