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. 2013 Jan 10;8(1):e53803. doi: 10.1371/journal.pone.0053803

Figure 4. Differential PTPMT1 knockdown alters the absolute level of HeLa cell apoptosis.

Figure 4

(A) HeLa cells were transfected with a dose response of non-targeting (blue line), PTPMT1#1 (green line) or PTPMT1#2 (orange line) siRNA over 120 hours. Cells were transfected with 0, 5, 10, 25, or 50 nM of each siRNA and real-time viability was tracked using xCELLigence. (B) Rate of change in cellular impedance, reflecting changes in proliferation (positive slope) and/or viability (negative slope) in cells transfected with control or PTPMT1 siRNAs. A lower rate of change in impedance is associated with decreased cellular attachment, indicative of cell death. Error bars indicate standard deviation of three experiments. Statistical significance was calculated using a student’s t test; * - p<0.05; ** - p<0.01; *** - p<0.001.