Skip to main content
. 2013 Jan 10;8(1):e53803. doi: 10.1371/journal.pone.0053803

Figure 8. Sublethal doses of alexidine dihydrochloride are sufficient to sensitize HeLa cells to the chemotherapeutic paclitaxel but are independent of PTPMT1 expression.

Figure 8

(A, B) HeLa cells were treated with 0.5 µM (A) or 1 µM (B) alexidine dihydrochloride, or a vehicle only control (DMSO, 0 µM condition, (A, B)) for 24 hours. Cells were then exposed to a dose response of paclitaxel for an additional 48 hours. Changes in viability were measured using Cell Titer Glo. (C) HeLa cells were treated with 0.5 µM or 1 µM alexidine dihydrochloride for 24 hrs before being exposed to 1 nM paclitaxel for an additional 24 hrs. Cell death was assayed by propidium iodide positivity. (D, E) HeLa cells were transfected with non-targeting siRNA (black circles), PTPMT1 siRNA#1 (grey squares), or PTPMT1 siRNA#2 (white triangles) for 30 hours before being exposed to a dose response of alexidine dihydrochloride for 24 hours. Viability was assayed using Cell Titer Glo. (F) Total ATP/cell was assayed in cells treated with alexidine dihydrochloride for 24 hrs. For each experiment, error bars indicate standard deviation of three experiments. Statistical significance was calculated using a student’s t test; * - p<0.05; ** - p<0.01; *** - p<0.001.