. 2012 Jul 25;8:120. doi: 10.1186/1746-6148-8-120

Table 1.

Primers and probes used in the rRT-PCR assays

rRT-PCR Assay Name	Target Genes (GenBank Accession Number)	Primer or Probe Names^a	Sequence 5' to 3' and Nucleotide Location	Length of Fragment (bp)
ERAV	5'-UTR of ERAV (L43052)	ERAV F	AGCGGCK^dTGCTGGATTTTC (397-415)	60
		ERAV R	CATY^eTGYCAGCTTGGTGACA (438-457)
		ERAV Pr	FAM^b-CGGTGCCATTGCT-MGB^c (417-429)
ERBV1	5'-UTR of ERBV1 (NC_003983)	ERBV1 F	CCCCTT^fCCCTGAAGATTGCT (148-167)	61
		ERBV1 R	GGCAAACGACCAACACATCA (190-209)
		ERBV1 Pr	FAM-TTCTTCCAACTAAACCC-MGB (169-185)
ERBV2	5'-UTR of ERBV2 (NC_003077)	ERBV2 F	CCCCAACCCTTGAGATTGCT (148-167)

^aF = Forward primer. R = Reverse primer. Pr = Probe.

^bReporter dye (FAM; 6-carboxyfluorescein) labeled nucleotide.

^cNonfluorescent quencher dye (MGB™; minor groove binding) labeled nucleotide.

^dK represents G or T.

^eY represents C or T.

^f Nucleotide difference between the forward primers of ERBV1 and ERBV2 are in bold.