Table 3.
Primers used in the cRT-PCR assays
| cRT-PCR Assay Name | Primer Names | Sequence 5' to 3' and Nucleotide Location (nt) | Genbank Accession Number | Reference |
|---|---|---|---|---|
| ERAV 5′UTR |
ERAV 5′UTR F |
TCAGCCCCCTGTCATTGACT (341-360) |
NC_003982 |
This study |
| |
ERAV 5′UTR R |
TGRaTCAGGGCTGTAACCA (769-786) |
|
|
| ERAV Poly |
ERAV Poly F |
TGGATGAAGTGGTTTTTGC (6384-6402) |
|
|
| |
ERAV Poly R |
CAGTCAAAGCCTGGTTGTCA (6502-6521) |
|
|
| ERAV Poly2 |
ERAV Poly F |
TGGATGAAGTGGTTTTTGC (6384-6402) |
|
|
| |
ERAV Poly R2 |
ACTCTCATTGCATCAGCTGC (6977-6996) |
|
|
| ERBV 5′ UTR |
ERBV 5′UTR F |
TTTCGTTCCWbCTTTAGCRaGG (349-368) |
NC_003983 |
|
| |
ERBV 5′UTR R |
TCAGATCCGCACTCTATGAAG (764-784) |
|
|
| ERBV OUTER1 |
ERBV OUTER F |
TTTTGATGCTTCACATTCTCC (7986-8006) |
|
[27] |
| |
ERBV OUTER R |
CGCTGTACCCTCGGTCCTACTC (8746-8767) |
|
|
| ERBV OUTER2 |
ERBV OUTER F |
TTTTGATGCTTCACATTCTCC (7986-8006) |
|
|
| |
ERBV INNER R |
GCCTCGGCGAGTGAAGAG (8721-8739) |
|
|
| ERBV INNER1 |
ERBV INNER F |
CTTACTAYcGAATGTGARGGGGC (8117-8138) |
|
|
| |
ERBV INNER R |
GCCTCGGCGAGTGAAGAG (8721-8739) |
|
|
| ERBV INNER2 |
ERBV INNER F |
CTTACTAYGAATGTGARGGGGC (8117-8138) |
|
|
| |
ERBV OUTER R |
CGCTGTACCCTCGGTCCTACTC (8746-8767) |
|
|
| ERBV Poly1 |
ERBV Poly F |
TTGAGTTGACCCTTCTGCA (7409-7427) |
|
This study |
| |
ERBV Poly R |
TCATACTCTGAAATGRaKdTCCATTG (7530-7553) |
|
|
| ERBV Poly2 |
ERBV Poly F |
TTGAGTTGACCCTTCTGCA (7409-7427) |
|
|
| ERBV Poly R2 | GCTGAACCAATGCCTAATCC (7879-7898) |
a R represents A or G.
b W represents A or T.
c Y represents C or T.
d K represents G or T.