Table 1.Saccharomyces cerevisiae and Sordaria macrospora strains used in this study.
| Strain | Characteristics | Source |
|---|---|---|
| Sc PJ69-4A |
MATa; trp1-901; leu2-3,112; ura3-52; his3-200; ga14D; ga18OD; LYS2::GAL1-HIS3; GAL2-ADE2; met2::GAL7-lacZ |
James et al. (1996)38 |
| Sc WCG4a |
MATα; his3-11,15; leu2-3,112; ura3-52 |
M. Thumm, Göttingena |
| Sc atg8Δ |
MATα; his3-11,15; leu2-3,112; ura3-52; atg8Δ::KAN |
M. Thumm, Göttingena |
| Sc atg4∆ |
MATα; his3-11,15; leu2-3,112; ura3-52; atg4Δ::HIS5 |
M. Thumm, Göttingena |
| Sc Y187 |
MATα; ura3-52; his3-200; ade2-101; trp1-901; leu2-3, 112; gal4∆; met∆; gal80∆; MEL1; URA3::GAL1UAS-GAL1TATA-lacZ |
Clontech |
| Sc AH109 |
MATa; trp1-901; leu2-3, 112; ura3-52; his3-200; ade2-101; gal4∆, gal80∆; LYS2::GAL1UAS-GAL1TATA-HIS3; GAL2UASGAL2TATAAde2; URA3::MEL1UAS-MEL1TATA-lacZ; MEL1 |
Clontech |
|
S48977 |
Wild type |
U. Kück, Bochum,b |
|
S66001 |
∆ku70::natR |
Pöggeler and Kück (2006)67 |
|
S23442 |
mutation in fus gene, brownish ascospores |
Nowrousian et al. (2012)39 |
| Sc WCG4a + pRS426-met25 |
MATα; his3-11,15; leu2-3,112; ura3-52 + pRS426-met25 |
This study |
| Sc atg8Δ + pRS426-met25 |
MATα his3-11,15; leu2-3,112; ura3; atg8Δ::KAN; + pRS426-met25 |
This study |
| Sc atg8Δ + pRS315-EGFP-Atg8 |
MATα; his3-11,15; leu2-3,112; ura3-52; atg8Δ::KAN; egfp-atg8 under control of endogenous promoter |
This study |
| Sc atg8Δ + pRS-met-Smatg8 |
MATα; his3-11,15; leu2-3,112; ura3-52; atg8Δ::KAN; Smatg8 under control of met25 promoter |
This study |
| Sc atg4∆ + pRS426-met25 |
MATα; his3-11,15; leu2-3,112; ura3-52; atg4Δ::HIS5; + pRS426-met25 |
This study |
| Sc atg4∆ + pRS316-Atg4 |
MATα; his3-11,15; leu2-3,112; ura3-52; atg4Δ::HIS5; ATG4 under control of endogenous promoter |
This study |
| Sc atg4∆ + pRS-met-Smatg4 |
MATα; his3-11,15; leu2-3,112; ura3-52; atg4Δ::HIS5; Smatg4 under control of met25 promoter |
This study |
| S ∆Smatg8 |
∆Smatg8::hygR, ssi |
This study |
| S ∆Smatg8::Smatg8ect |
∆Smatg8::hygR, Smatg8ect, natR, ssi |
This study |
| S ∆Smatg8::egfp-Smatg8ect |
∆Smatg8::hygR, egfp-Smatg8ect, natR, ssi |
This study |
| S ∆Smatg8::egfp-Smatg8-DsRed-SKLect |
∆Smatg8::hygR, egfp-Smatg8-DsRed-SKLect, natR, ssi |
This study |
| S ∆Smatg8::DsRed-SKLect |
∆Smatg8::hygR, DsRed-SKLect, natR |
This study |
| S ∆Smatg4 |
∆Smatg4::hygR, ssi |
This study |
| S ∆Smatg4::Smatg4ect |
∆Smatg4::hygR, Smatg4ect, natR, ssi |
This study |
| S ∆Smatg4::egfp-Smatg8ect |
∆Smatg4::hygR, egfp-Smatg8ect, natR |
This study |
| S ∆Smatg4::Smatg8-egfpect |
∆Smatg4::hygR, Smatg8-egfpect, natR |
This study |
| S ∆Smatg4::egfp-Smatg8G116ect |
∆Smatg4::hygR, egfp-Smatg8G116ect, natR |
This study |
| S ∆Smatg4::Smatg4-egfpect |
∆Smatg4::hygR, Smatg4-egfpect, natR, ssi |
This study |
| S ∆Smatg4::DsRed-SKLect |
∆Smatg4::hygR, DsRed-SKLect, natR |
This study |
| S wt::Smatg8-egfpect |
Smatg8-egfpect, natR, ssi |
This study |
| S wt::egfp-Smatg8mutect | egfp-Smatg8mutect, natR | This study |
Sc, Saccharomyces cerevisiae; S, Sordaria macrospora; hygR, hygromycin resistant; natR, nourseothricin resistant; ssi, single spore isolate. aDepartment of Biochemistry II, UMG, Georg-August University Göttingen, Germany bDepartment of General and Molecular Botany, Ruhr-University Bochum, Germany