Figure 4. The indicated CRC cells were cultured for 48h in the presence of Dmso, SB202190 (10 μM), sorafenib (20 μM) or a combination of SB202190 (10 μM) and sorafenib (20 μM). Then, cells were harvested and subjected to immunoblot with the indicated antibodies (A, B, D, E, F), or living cells were stained with Coomassie blue (C). To inhibit caspase 8 activity, Z-IEDT FMK (10 μM) was added to the culture for the last 12h (F).