Figure 1. (A) Protective effect of 10 mM alanyl-glutamine (NFV+AQ) supplementation on IEC-6 cell migration following 1 h incubation with 70 μg/mL of nelfinavir (NFV) at 12 and 24 h. After reaching confluency, IEC-6 monolayers were scratched, wells were incubated with 70 μg/mL of NFV for 1h and washed with media without glutamine, followed by incubation with 10mM of AQ. The bars represent means ± SE for the number of migrating cells per square millimeter of scraped area. *p < 0.05, compared with control group with media without glutamine, by student’s unpaired t test. ^#p < 0.05, compared with group with NFV, by student’s unpaired t test. (B) Representative images of migration of IEC-6 cells at 24 h from the control group, NFV group, and AQ supplemented group, following NFV exposure for 1 h. Diagram shows scraping area with grid (each square = 0.1 mm²), overlapping the column of the farthest migration. The IEC-6 cells were tracked by traced dots for counting. The dots were counted digitally by Image Pro Plus software.