Figure 2. (A) Protective effect of supplementation with 10mM of alanyl-glutamine (AQ) on IEC-6 cell proliferation following 1h of exposure to nelfinavir (NFV) at 70μg/mL . (B) Effect of 1, 5, 10 and 50mM of AQ supplementation on IEC-6 cell proliferation, evaluated with a colorimetric assay by detecting absorbance using an ELISA microplate-reader at 450 nm. After 24 and 48 h, wells were incubated for 4 h with 10 μL of tetrazolium salt and the absorbance was measured. After 24 and 48 h, wells were incubated for 4 h with 10 μL of tetrazolium salt and the absorbance was measured. Values are expressed as mean ± standard error. *p < 0.05, compared with control group with media without glutamine, by student’s unpaired t test. ^#p < 0.05, compared with group with NFV, by student’s unpaired t-test.