Figure 5. (A) Effect of nelfinavir at 100 mg/kg (NFV) and supplementation with 10 mM of alanyl-glutamine (NFV+AQ) on jejunum intestinal morphometry and mitotic index compared with control group (CONTROL). Values are expressed as percentage of control. (B) Effects of NFV and AQ supplementation (NFV+AQ) on cell death in the jejunum after 7 d of treatment compared with the PBS control (C). For each animal (n = 6), intestinal samples were collected and stained with TUNEL for immunohistochemistry. All slices were counterstained with methyl green, designed for nuclear counterstaining (stained light green, similar to blue color), which provide excellent contrast to brown. A strong methyl green stain, observed in the negative control slice, means that no immunostaining was detected, as expected. Negative control represents a sample of the jejunum where the antibody was replaced by 5% PBS/BSA. *p < 0.05, compared with PBS control group, by student’s unpaired t test. ^#p < 0.05, compared with group with NFV, by one-way ANOVA, with Bonferroni’s post-test.