Figure 7. Effects of Dmab and ZOL on HUVEC invasion. Cultured HUVECs were harvested and suspended in DMEM and incubated for 24 h on filter coated with Matrigel in the absence or in presence of Dmab or ZOL; 3 mL of DMEM supplemented with 10% FBS (positive control) or not (negative control) was added to the well underneath the insert. Cells that crossed the Matrigel-coated filter were fixed, stained and counted. Six random microscope fields were counted for each group. Statistical analysis: p < 0.01 control vs. 5μM ZOL treated cells; p < 0.001 control vs. 20 μM and 80 μM ZOL treated cells.