Table 3.

Orphan snoRNA genes (C/D and H/ACA) in E. histolytica

snoRNA genes	Len	Seq	Modification	Antisense element	Scaffold	Start	End	Homology Yeast Human	Location
	(nt)	(%)
EhCDOrph1	95	95%	unknown	unknown	DS571162	42554	42648	unknown	EHI_155390 (+)
EhCDOrph2	87	94%	unknown	unknown	DS571301	21222	21308	unknown	IR
EhCDOrph3	107	94%	unknown	unknown	DS571358	4592	4698	unknown	IR
EhCDOrph4	91	96%	unknown	unknown	DS571422	5594	5684	unknown	IR
EhCDOrph5	84	94%	unknown	unknown	DS571468	9619	9702	unknown	IR
EhCDOrph6	94	--	unknown	unknown	DS571178	12358	12451	unknown	3'UTR/IR
EhCDOrph7	94	--	unknown	unknown	DS571178	13726	13819	unknown	3'UTR/IR
EhACAOrph1	115	91%	unknown	unknown	DS571172	5407	5293	unknown	IR
EhACAOrph2	135	93%	unknown	unknown	DS571155	108854	108988	unknown	IR/5’UTR ●
**EhACAOrph3	137	94%	unknown	unknown	DS571258	10028	9892	unknown	IR
EhACAOrph4	122	90%	unknown	unknown	DS571205	43143	43022	unknown	IR
EhACAOrph5	129	-	unknown	unknown	DS571332	15845	15717	unknown	IR
**EhACAOrph6	158	-	unknown	unknown	DS571298	19208	19365	unknown	IR
EhACAOrph7	130	-	unknown	unknown	DS571219	6608	6737	unknown	IR
EhACAOrph8	131	88%	unknown	unknown	DS571162	44597	44467	unknown	IR ●
EhACAOrph9	120	89%	unknown	unknown	DS571164	102500	102619	unknown	IR ●
EhACAOrph10	149	87%	unknown	unknown	DS571179	6844	6696	unknown	EHI_093690 (−) ●
EhACAOrph11	139	94%	unknown	unknown	DS571299	12352	12214	unknown	EHI_099700 (−)
EhACAOrph12	134	91%	unknown	unknown	DS571402	6404	6271	unknown	EHI_067510 (−) ●
**EhACAOrph13	137	95%	unknown	unknown	DS571501	3747	3883	unknown	EHI_171990 (+)
**EhACAOrph14	153	97%	unknown	unknown	DS571295	13935	14087	unknown	EHI_082520 (−)
*EhACAOrph15	148	91%	unknown	unknown	DS571166	95075	95222	unknown	EHI_127390 (−)

** snoRNAs validated by RT-PCR and Northern, * validated only by RT-PCR.

Note: “Len.” denotes length of the snoRNA genes, “Seq.” is sequence identity of corresponding snoRNA genes in E. dispar, “IR”, intergenic region, “NA”, no annotation. snoRNA located close to ribosomal protein genes ●. (+) and (−) represents snoRNA in sense and antisense orientation with respect to host gene.