Table 1. Primer sequences used in this study.
Primer | Sequence (5′-3′) | Gene | Amplicon size (bp) |
P1U | ATCTGCATGGCCCACACGGTC | SMP integrase | 823 |
P1L | CAAACGGACCTCTATCGCCTC | ||
P2U | TCGTCGTCTTCGGTTAGG | SMP repressor | 647 |
P2L | TTGAGGTTTGGGTCTGTC | ||
P3U | TAGCGGTGCTGGTGGTCATAC | SMP lyase | 817 |
P3L | TCCACCCTCGCAAGCTGGTCT | ||
P4U | TGCAGGCAAAGTACGTTTCCA | SMP major tail protein | 248 |
P4L | CTTCATGTTGTCGCTTGCTAC | ||
P5U | TGATGAAGCTGGTCTACTTGG | SMP head protein | 456 |
P5L | TCCATTGTGGCACGGATAGCA | ||
Lys-Gspa | TCGGAGCGTCAAGCCGTCTGAAAGC | ||
Lys-Gspb | GCAGAAAATGGATCATTGGACAAGT | ||
Lys-Gspc | CAAAATCGAAGTAACCATTGAGGGG | ||
Int-Gspa | AGCCACATCTTCCGCCATACCCTTG | ||
Int-Gspb | TCTAGCAGAGAACAGAGTTCCCTTG | ||
Int-Gspc | ACCCAAATTTACACCCATGTCACAA |