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PCR screening of the deletion in the hasV gene with Anabaena sp. 90 cells originating from different years. PCR analysis of genomic DNA demonstrates occurrences of the deletion in hasV in 2000, 2003, 2006 and 2009. PCRs with primers across the site of deletion show that the deletion appeared in 2006 (arrow). Recent cultures of Anabaena sp. 90 carry a deletion in hasV and this strain no longer produces hassallidin
