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. 2013 Jan 10;8(1):e53394. doi: 10.1371/journal.pone.0053394

Figure 4. TSA removal completely reverts the transcriptional changes observed after HDAC inhibition.

Figure 4

SH-SY5Y neuroblastoma cells were treated with vehicle or 250 nM TSA for 16 h, or with 250 nM TSA for 16 h and additionally 24 h without TSA (washout). mRNA transcript levels of HMGCR, MVK, LDLR, SREBP2 and ABCA1 were analyzed by real-time RT-PCR. Values were normalized to the internal standard β-actin and expressed as fold change relative to vehicle-treated cells. Values represent means ± SEM from at least three individual experiments (* p<0.05, *** p<0.001).